Rho dependent Termination
● This type of termination occur in Prokaryotes for termination of transcription. ◇ Transcription = Synthesis of RNA from DNA. ● Dna which have such a termination sequence which terminates the transcription only in presence of rho factor are called rho dependent termination. ● This termination sequence is rich in C but devoid of G .This types of sequence is necessary for function rho protein . ● Rho protein binds with mRNA which is elongated by rna polymerase at rut site (rho utilization site) and then rho protein moves from 5' to 3' end . ● When RNA polymerase reaches rho dependent termination sequence then rho protein shows its helicase activity and unwind DNA-RNA hybrid. ● This will disrupt and terminate transcription bubble and new synthesized mRNA is released. (Helicase is a enzyme that can unwind the dna strand or we can say breaks H bonds present in between the double helix of DNA )